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We are excited to share some important news regarding the HemEX™-Type9A mouse HSC expansion basal medium. In CSTI’s ongoing commitment to excellence and customer satisfaction, upgrades have been made to this product. The upgraded version, HemEX™-Type9AΦ, now available for purchase, not only increases the proliferation of undifferentiated HSCs compared to the previous version, but it is also now possible for the expansion from a single HSC. HemEX™-Type9AΦ is available for purchase.
Please note that the previous version, HemEX™-Type9A, is still in stock, and transition to the new version in March 2025 or when the existing stock is depleted. If you require the previous version to maintain consistency in your data, please let us know as soon as possible how much you would need, and we will do our best to secure it for you.
We appreciate your continued trust and support, and we are confident that these upgrades will significantly enhance your experience with the product.
Comparison between HemEX™-Type9A vs HemEX™-Type9AΦ
Product Name Catalog# | HemEX™-Type9AΦ New A5P10P01C | HemEX™-Type 9A A5P00P01C |
Albumin Substitute | Soluplus® | PVA (polyvinyl alcohol) |
Proliferation of undifferentiated HSC | Increased1) Fig.S3A | Higher compared to conventional media |
Expansion from single HSC | Yes | No |
Ratio of HSC to HSPC during culture | Higher (maintains more CD201+/CD150+1)Fig.S3E) | Higher compared to conventional media |
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A) Expansion of murine HSCs for 7 days with various albumin replacement polymer.
(B) Murine TPO ELISA. Ham’s F12 with 100 ng/ml Thrombopoietin (TPO) was supplemented with the indicated polymers and cultured for 3 days. TPO levels were significantly higher if Soluplus is present compared with plain or PVA-supplemented medium.
(D) Percentage of viable cells in HSC colonies grown from freshly isolated single CD34-CD150+KSL cells after 19 days of culture in PVA (n=288)- and Soluplus (n=290)-based media (flow cytometry ). Representative FACS plots of single-cell derived colonies (day 19).
References:
1. Becker, H. J. et al. Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion. Cell Stem Cell 30, 987-1000.e8 (2023) doi: 10.1016/j.stem.2023.06.002.