BIOMIMESYS® Brain
3D cell culture model for Brain
Product Basics
BIOMIMESYS® Brain is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold’s composition and mechanical properties can mimics the microenvironment of brain tissues. It is proven to provide better in vitro/in vivo correlation than 2D models.
Key Features
- Mimics the microenvironment of brain tissue
- Better in vitro/in vivo correlation
- Ready to use
- Applicable for most downstream applications
- Batch to Batch consistent
Technical Information
Mimics the Microenvironment of Brain Tissue
By mimicking the low stiffness of brain tissues, BIOMIMESYS® Brain allows to grow mini-brains (spheroids of brain cells) or neuronal networks in 3D.
For a better in vitro/in vivo correlation
By using a brain-specific ECM with BIOMIMESYS® Brain, Luhmes cells showed lower sensitivity to Parkinson’s disease inducers in BIOMIMESYS® compared to 2D culture.
Ready to Use
BIOMIMESYS® Oncology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
Successfully Tested Cells
Cell Lines
Human brain metastasis: SA87
Human neuroblastoma : SH-SY5Y
Human glioblastoma : CB109 / CB74 / CB191
Human gliome : DIPG007
Normal human neurons : Luhmes
Normal human colon fibroblast : CCD18-co
Normal mouse preadipocytes : 3T3-L1 / 3T3-F442A
Human colorectal adenocarcinoma : HCT 116
Human liver hepatocellular carcinoma
Primary Cells
Human astrocytes : cryopreserved
Mouse neuronal cells
Stem Cells
Human induced pluripotent stem cells : CNS4U, Peri4U
Complete List of Tested Cells [PDF]
Compatible with Most Downstream Applications
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications.
Specification
Composition:
- Hyaluronic acid (HA) (1.6MDa) grafted with RGDS
- Adipic acid dihydrazide (ADH) as crosslinker
- Collagen Type IV
- cationic bioplolymer
Physicochemical Features:
- Young’s modulus: ~0.1kPa – different stiffness is available through customization
- Porosity: 150±30μ
Pricing
BIOMIMESYS® Brain
- 3D cell culture model for Brain
24 Hydrogel / 96 Well
Transparent Plate
- SKU: BIO_BRA_96_24_transp
- Price:
$410.00→ $369.00
Black Plate
- SKU: BIO_BRA_96_24_black
- Price:
$410.00→ $369.00
96 Hydrogel / 96 Well
Transparent Plate
- SKU: BIO_BRA_96_96_transp
- Price:
$590.00→ $531.00
Black Plate
- SKU: BIO_BRA_96_96_black
- Price:
$590.00→ $531.00
References & Literature
De Conto, V. et al. In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells. Toxicology in Vitro 77, 105235 (2021) doi: 10.1016/j.tiv.2021.105235.
Other Documents
- Retrieve Cells / Washing Gels
- First Steps - Co-culture, Change Medium, Seeding
- Protocol for Metabolic Activity Determination
- RNA or DNA Extraction
- Protein Extraction
- Microscopy
- Flow Cytometry
- Preparation of Samples for Histological Labeling
- Evaluation of BIOMIMESYS® hydroscaffold in mice bearing subcutaneous HCT 116 Human Colorectal tumour cells
- Technical Datasheet BIOMIMESYS® Brain
- MSDS BIOMIMESYS® Brain
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES