BIOMIMESYS® Liver
3D cell culture model for Liver
Product Basics
BIOMIMESYS® Liver is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold’s composition and mechanical properties can mimics the microenvironment of Liver. It is proven to provide better in vitro/in vivo correlation and cell longevity than 2D models.
Key Features
- Mimics the microenvironment of Liver
- Better in vitro/in vivo correlation
- Better cell longevity and proper phenotype
- Ready to use
- Applicable for most downstream applications
- Batch to Batch consistent
Technical Information
For a better cell longevity and a proper phenotype
BIOMIMESYS® Liver is dedicated to the culture of liver cells such as hepatocytes. Unlike in 2D culture, primary human hepatocytes in 3D within BIOMIMESYS® Liver had their enzymatic activities (e.g. CYPs) and albumin secretion maintained until 21 days.
For a better in vitro/in vivo correlation
Ready to Use
BIOMIMESYS® Oncology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
Successfully Tested Cells
Cell Lines
Human hepatocytes : HepaRG, IHH
Human stellate : LX2
Human liver hepatocellular carcinoma : HepG2
Human liver hepatoma : PLC / PRF-5
Human colorectal adenocarcinoma : HCT 116
Primary Cells
Human hepatocytes : HH cryopreserved
Stem Cells
Hematopoietic : CD34+
Human induced pluripotent stem cells : hiPS
Complete List of Tested Cells [PDF]
* Proof of Concept:
・Increased Culture Time [PDF]
・Maintain Hepatocyte Functions [PDF]
・Maintain Cyp Activity & Inducibility [PDF]
・Allows Hepatotoxicity Studies [PDF]
Compatible with Most Downstream Applications
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications.
Specification
- Hyaluronic acid (HA) (1.6MDa) grafted with RGDS
- Adipic acid dihydrazide (ADH) as crosslinker
- Collagen Type I & VI
- Galactosamine
- Young’s modulus: ~0.6kPa – different stiffness is available through customization
- Porosity: 117±23μm
Pricing
BIOMIMESYS® Liver
- 3D cell culture model for Liver
24 Hydrogel / 96 Well
Transparent Plate
- SKU: BIO_LIV_96_24_transp
- Price:
$410.00→ $369.00
Black Plate
- SKU: BIO_LIV_96_24_black
- Price:
$410.00→ $369.00
96 Hydrogel / 96 Well
Transparent Plate
- SKU: BIO_LIV_96_96_transp
- Price:
$590.00→ $531.00
Black Plate
- SKU: BIO_LIV_96_96_black
- Price:
$590.00→ $531.00
384 Hydrogel / 384 Well / Black
1 Plate
- SKU: BIO_LIV_384_384_black
- Price:
$665.00→ $600.00
5 Plates
- SKU: BIO_LIV_384_384_black_5
- Price:
$2500.00→ $2250.00
References & Literature
1. Bachmann, A. et al. 3D Cultivation Techniques for Primary Human Hepatocytes. Microarrays (Basel) 4, 64–83 (2015) doi: 10.3390/microarrays4010064
2. Wilkening, S., Stahl, F. & Bader, A. Comparison of primary human hepatocytes and hepatoma cell line Hepg2 with regard to their biotransformation properties. Drug Metab Dispos 31, 1035–1042 (2003) doi: 10.1124/dmd.31.8.1035.
Other Documents
- Retrieve Cells / Washing Gels
- First Steps - Co-culture, Change Medium, Seeding
- Protocol for Metabolic Activity Determination
- RNA or DNA Extraction
- Protein Extraction
- Microscopy
- Flow Cytometry
- Preparation of Samples for Histological Labeling
- Evaluation of BIOMIMESYS® hydroscaffold in mice bearing subcutaneous HCT 116 Human Colorectal tumour cells
- Technical Datasheet BIOMIMESYS® Liver
- MSDS BIOMIMESYS® Liver
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES