ALyS™505NK series Natural Killer (NK) Cells

ALyS™505NK Series

Serum-free cell culture medium for activation and expansion of Human NK cells

Product Basics

ALyS505NK-AC and ALyS505NK-EX are serum-free, xeno-free cell culture media optimized for human natural killer (NK) cells derived from peripheral blood mononuclear cells (PBMCs). NK cells are crucial for innate immunity. Unlike T cells, they can damage virus-infected cells and cancer cells without prior sensitization.
Many applications necessitate the expansion of NK cells. However, in vitro cultivation of NK cells often results in a low expansion rate, an exhausted phenotype due to long-term expansion, and overgrowth of T cells if present in the initial culture. By employing ALyS505NK-AC for activation and ALyS505NK-EX for expansion, one can attain a highly pure, NK-rich population of cells in 14 days without the need for sorting with beads or flow cytometry.
Artificial Serum is recommended for the primary culture derived from PBMC. Both ALyS505NK-AC and ALyS505NK-EX are available with or without IL-2 (1000IU/mL).

Key Features

Serum-free (xeno-free) culture medium
Facilitates efficient and high proliferation of NK cells.
Enables the attainment of a higher ratio of NK cells to T cells
Expanded NK cells exhibit low cytotoxic activity at a given E/T ratio
Available with or without IL-2 (1000IU/mL)
Culture bag format for larger sizes is available for ALyS505NK-EX
Optimal for NK cell therapy

Technical Information

Robust Proliferation

As shown in Fig. 1, the proliferation of PBMC cultured in ALyS505NK-AC & EX over 14 days was higher than that in competitor G’s medium.

Highly efficient proliferation ratio of NK cells

In Fig. 2 and 3, PBMCs were collected from three donors and cultured with ALyS505NK-AC & EX and competitor G’s medium. As depicted in Fig. 2, the number of NK cells obtained on day 14 was significantly higher when cultured with ALyS505NK-AC & EX. Furthermore, as shown in Fig. 3, the ratio of NK cells to T cells was between 55-75% and 45-25% with ALyS505NK-AC & EX. In contrast, only 25-45% of NK cells were obtained after culturing in competitor G’s medium. Despite some noticeable variation among donors, ALyS505NK-AC & EX demonstrated highly efficient proliferation of NK cells and a high NK cell ratio.

Phenotype of NK cells are maintained

In Fig. 4, the cytotoxicity level of NK cells was evaluated by incubating them with K562 for four hours at varying E/T ratios. The NK cells cultured from ALyS505NK-EX exhibited cytotoxic activity even at low E/T ratios.

Specification

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SKU	Product	Description	Size	Contents	Storage
01600P02	ALyS505NK-AC	IL-2 free (Activation)	200ml	Bottle	4°C
01610P02	ALyS505NK-AC1000	IL-2 concentration 1,000lU/mL (Activation)	200ml	Bottle	4°C
01400P10/01400C10	ALyS505NK-EX	IL-2 free (Expansion)	1L	Bottle/Culture Bag	4°C
01410P10/01410C10	ALyS505NK-EX1000	IL-2 concentration 1,000lU/mL (Expansion)	1L	Bottle/Culture Bag	4°C

Manufactured by: Cell Science and Technology Institute

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