CiMS™
Serum-free culture medium for human MSC.
Animal-free or Xeno-free supplement available to use with base medium
Product Basics
CiMS™ is a serum-free cell culture medium for human mesenchymal stem cells (hMSC). The CiMS™-BM (base medium) is optimized for multiple passage expansions of various undifferentiated hMSCs. Pre-coating of culture vessels with an attachment matrix is not required, thereby improving efficiency. Furthermore, by adding either an animal-free supplement (CiMS™-sAF) or a xeno-free supplement (CiMS™-sXF), which are optimized for CiMS™-BM, long-term serum-free culture with high proliferation can be achieved.
Key Features
- Serum-free culture medium designed for hMSC
- Facilitates the expansion of various hMSCs in an undifferentiated state
- Eliminates the need for pre-coating culture vessels, thereby improving efficiency
- Offers optimized animal-free (CiMS™-sAF) or xeno-free (CiMS™-sXF) supplements
- Enables long-term culture with high proliferation of various hMSCs
- Allows differentiation into several desired lineages
- Manufactured in compliance with Japanese GMP guidelines
Technical Information
Fig. 1
hMSC derived from adipose tissue (AT-MSCs) cultured in CiMS™-BM supplemented with CiMS™-sAF, maintained normal morphology and adherence to the culture dish (day 4 shown).
Fig. 2
Proliferation activity of AT-MSCs was evaluated by population doubling level (PDL). Cells cultured in CiMS™-BM supplemented with either CiMS™-sXF or CiMS™-sAF without matrices have shown high proliferation through passages. Results are similar to cells cultured in MSC-T4 with fibronectin coated plates.
Fig. 3
Osteogenic, adipogenic and chondrogenic differentiation capability of AT-MSCs cultured in CiMS™-BM with CiMS™-sAF supplement were confirmed by Alizarin Red S staining, Oil Red O staining, and toluidine blue staining, respectively. The same differentiation capability was confirmed with CiMS™-sXF has been confirmed.
Specification
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| Cat# | Product | Description | Size | Contents | Storage Upon Arrival |
|---|---|---|---|---|---|
| A2G00P05C | CiMS™-BM | Basal medium | 500ml | 1 bottle | 4°C, protect from light |
| A2G10P1CC | CiMS™-sXF | Xeno-free supplement | 10ml | 1 bottle | Below -20°C |
| A2G20P1CC | CiMS™-sAF | Animal-free supplement | 10ml | 1 bottle | Below -20°C |
- Manufactured by: Cell Science and Technology Institute
Pricing
CiMS™
- Serum-free culture medium for human MSC
- Animal-free or Xeno-free supplement available to use with base medium
CiMS™-BM
Base Medium
- SKU: A2G00P05C
- Size: 500ml
- Price:
$200.00→ $180.00
CiMS™-SXF
Xeno Free Supplement
- SKU: A2G10P1CC
- Size: 10ml
- Price:
$500.00→ $450.00
CiMS™-SAF
Animal Free Supplement
- SKU: A2G20P1CC
- Size: 10ml
- Price:
$775.00→ $695.00
References
- Sato, K. et al. Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology – Animal 51, 515–529 (2015) doi: 10.1007/s11626-014-9860-0.
- Murabayashi, D., Mochizuki, M., Tamaki, Y. & Nakahara, T. Practical methods for handling human periodontal ligament stem cells in serum-free and serum-containing culture conditions under hypoxia: implications for regenerative medicine. Hum Cell 30, 169–180 (2017) doi: 10.1007/s13577-017-0161-2.
Other Documents
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES