HemEx™-TypeH
Human HSC expansion basal medium
Product Basics
HemEx™-TypeH (human HSC expansion basal medium) is specially formulated for expansion of human hematopoietic stem cells (HSC). Based on Iscove’s MDM, it can expand long-term HSCs (LT-HSCs) from umbilical cord blood-derived (CB) hHSCs and peripheral blood stem cells (PBSCs). This product contains only Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) as a supplement, allowing users the flexibility to combine cytokines and small molecules.
Contaminants derived from albumin can trigger the differentiation of HSC. By replacing albumin with polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (PCL-PVAc-PEG, Soluplus), HemEx-TypeH can maintain HSCs in an undifferentiated state for over one month of expansion culture.
Key Features
- Serum free. Albumin free
- Chemically defined
- Verified proliferation of human HSC and hematopoietic stem and progenitor cell (HSPC)*
- Development was supervised by Dr. Satoshi Yamazaki (Tokyo University, Japan)
Technical Information
Maintaining the Undifferentiated State
HemEx™ -TypeH utilizes PCL-PVAc-PEG instead of albumin to suppress the differentiation of HSCs.
Fig.1 Human HSCs expansion after 10 days in complete HemEx™ -TypeH.
- Umbilical cord blood-derived (CB) hHSCs cultured with HemEx™ -TypeH proliferated well for 10 days.
- When the cultured human HSC cells were analyzed by flow cytometry, it was confirmed that the characteristics of human HSC were maintained.
*Requires the addition of cytokines
Specification
- Size: 100mL
- Storage temperature: 4°C
- Shelf life: To be determined
- Manufactured by: Cell Science and Technology Institute
Pricing
HemEx™ - TypeH
- Human HSC expansion basal medium
- SKU: A2D10P01C
- Size: 100mL
- Price: $320.00
References
- Sakurai, M. et al. Chemically defined cytokine-free expansion of human haematopoietic stem cells. Nature 615, 127–133 (2023) doi: 10.1038/s41586-023-05739-9.
- Sakurai, M., Ishitsuka, K. & Yamazaki, S. Chemically defined cytokine-free expansion of human haematopoietic stem cells. Protocol Exchange (2023) doi: 10.21203/rs.3.pex-2163/v1.
- Ishitsuka, K., Nishikii, H., Kimura, T., Sugiyama-Finnis, A. & Yamazaki, S. Purging myeloma cell contaminants and simultaneous expansion of peripheral blood-mobilized stem cells. Experimental Hematology 131, 104138 (2024) doi: 10.1016/j.exphem.2023.104138.
Other Documents
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES