PeptiGrowth PG-003
BDNF Alternative Peptide (TrkB agonist)
Product Basics
Brain-derived neurotrophic factor (BDNF) binds to the TrkB receptor, regulating neuronal growth, survival, and synaptic function.
PG-003 exhibits agonistic activity against the TrkB receptor by specifically binding to and inducing dimerization and promoting cell signaling pathways. It exhibits comparable activity at 1/2.5 of the concentration of recombinant BDNF due to a difference in molecular weight and does not remain in the final product.
Key Features
- Alternative to Brain-Derived Neurotrophic Factor (BDNF)
- Comparable activity at 1/2.5 of the concentration of rBDNF*
- Supports survival and differentiation of nervous system cells
- Stable. >80% remained after 14 days in serum free media at 37˚C
- Does not remain in the final products
- Fully synthetic and animal-free
GMP grade can be manufactured upon request.
*Please note that this figure is an example and concentration optimization is needed.
Technical Information
(1) PG-003 showed physiological activity equivalent to BDNF at 1/2.5 the concentration of BDNF
PeptiGrowth Inc. conducted a functional comparison test between their BDNF Alternative Peptide (PG-003) and the commercially available recombinant BDNF. From the results, PG-003 exhibited properties that promote the phosphorylation of the TrkB receptor and the expression of the NFAT-responsive reporter gene at levels equivalent to recombinant BDNF on a per-molecule basis. The molecular weight of PG-003 is approximately 5.1 kDa, which is approximately 2.5 times smaller than that of recombinant BDNF. Therefore, on a mass concentration basis (ng/mL), PG-003 demonstrates comparable activity at 2.5 times less concentration than recombinant BDNF.
(2) Neurite outgrowth was induced by treating SH-SY5Y cells (human neuroblastoma-derived cells) with equimolar concentrations of rBDNF or PG-003
Figure 1: Fluorescence Microscopic Images (5×) of Neurite Outgrowth of Human Neuroblastoma Cell (SH-SY5Y)
Figure 2: Fluorescence Microscopic Images (10×) of Neurite Outgrowth of Human Neuroblastoma Cell (SH-SY5Y)
Neurite development induced by PG-003 and rBDNF
Figure 3: Quantification of neurite development behavior of SH-SYSY cells by the effect of PG-003 or rBDNF
(3) Growth promotion of neural axon using Nerve Organoid
Jiksak Bioengineering Inc. evaluated the behavior of neurite bundle elongation using their proprietary technology, Nerve Organoid™, with BDNF or PG-003. As a result, when PG-003 was used, thicker and longer neurite bundles were formed.
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Specification
- Storage temperature: -20°C (Ship at 4°C)
- Shelf life: 2 years (To be extended)
- Manufactured by: PeptiGrowth Inc.
Pricing
10ug
- SKU: PG-003-10ug
- Size: 10ug (eq. ~25ug of Recombinant rBDNF)
- Price: $360.00
100ug
- SKU: PG-003-100ug
- Size: 100ug (eq. ~250ug of Recombinant rBDNF)
- Price: $1,500.00
1mg
- SKU: PG-003-1mg
- Size: 1mg (eq. ~2500ug of Recombinant rBDNF)
- Price: $10,800.00
References
- Binder, D. K. & and Scharfman, H. E. Mini Review. Growth Factors 22, 123–131 (2004) doi: 10.1080/08977190410001723308.
- Brunoni, A. R., Lopes, M. & Fregni, F. A systematic review and meta-analysis of clinical studies on major depression and BDNF levels: implications for the role of neuroplasticity in depression. International Journal of Neuropsychopharmacology 11, 1169–1180 (2008) doi: 10.1017/S1461145708009309.
- Tapia-Arancibia, L., Aliaga, E., Silhol, M. & Arancibia, S. New insights into brain BDNF function in normal aging and Alzheimer disease. Brain Research Reviews 59, 201–220 (2008) doi: 10.1016/j.brainresrev.2008.07.007.
- Bruggeman, K. F. et al. Temporally controlled growth factor delivery from a self-assembling peptide hydrogel and electrospun nanofibre composite scaffold. Nanoscale 9, 13661–13669 (2017) doi: 10.1039/C7NR05004F.
- A growing market for synthetically produced peptides. Nature (2022). https://www.nature.com/articles/d42473-022-00352-2
- Hu, T. et al. Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers. Molecular Biology Reports 51, 1143 (2024) doi: 10.1007/s11033-024-10044-3.
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES